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DuPont de Nemours random primer extension kit
Random Primer Extension Kit, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+primer+extension+kit/random+primer+extension+kit/10__1111_slash_j__1399___3054__2000__1100316__x-70-8-12
Average 90 stars, based on 1 article reviews
random primer extension kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Agarose Gel Electrophoresis:

Article Title: MIDA1, a protein associated with Id, regulates cell growth.
Article Snippet: .. Printed in U.S.A. 24818 at U niv of St A ndrew s on M arch 6, 2015 http://w w w .jbc.org/ D ow nloaded from tured 1% agarose gel, transferred to a nylon membrane, and hybridized with cDNA probe radiolabeled with a random primer extension kit (E. I. du Pont de Nemours & Co., Inc.) in the hybridization buffer (50% formamide, 5 3 SSC, 1 3 FBP (1% Ficol, 1% polyvinylpyrrolidone, 1% bovine serum albumin), 20 mM NaHPO3 pH 6.5, 100 mg/ml of salmon sperm DNA, 10% dextran sulfate, 0.1% SDS). ..

Article Title: Regulatory Function of Delta/YY-1 on the Locus Control Region-like Sequence of Mouse Glycophorin Gene in Erythroleukemia Cells
Article Snippet: .. Ten mg of RNA from each sample were electrophoresed in a denatured 1% gel agarose gel, transferred to a nylon membrane, and hybridized with a mouse glycophorin cDNA probe (1) radiolabeled with a random primer extension kit (DuPont) in the hybridization buffer (50% formamide, 5 3 SSC, 1 3 Denhardt’s reagent, 20 mM NaHPO3, pH 6.5, 100 mg/ml salmon sperm DNA, 10% dextran sulfate, 0.1% SDS). ..

Membrane:

Article Title: MIDA1, a protein associated with Id, regulates cell growth.
Article Snippet: .. Printed in U.S.A. 24818 at U niv of St A ndrew s on M arch 6, 2015 http://w w w .jbc.org/ D ow nloaded from tured 1% agarose gel, transferred to a nylon membrane, and hybridized with cDNA probe radiolabeled with a random primer extension kit (E. I. du Pont de Nemours & Co., Inc.) in the hybridization buffer (50% formamide, 5 3 SSC, 1 3 FBP (1% Ficol, 1% polyvinylpyrrolidone, 1% bovine serum albumin), 20 mM NaHPO3 pH 6.5, 100 mg/ml of salmon sperm DNA, 10% dextran sulfate, 0.1% SDS). ..

Article Title: Regulatory Function of Delta/YY-1 on the Locus Control Region-like Sequence of Mouse Glycophorin Gene in Erythroleukemia Cells
Article Snippet: .. Ten mg of RNA from each sample were electrophoresed in a denatured 1% gel agarose gel, transferred to a nylon membrane, and hybridized with a mouse glycophorin cDNA probe (1) radiolabeled with a random primer extension kit (DuPont) in the hybridization buffer (50% formamide, 5 3 SSC, 1 3 Denhardt’s reagent, 20 mM NaHPO3, pH 6.5, 100 mg/ml salmon sperm DNA, 10% dextran sulfate, 0.1% SDS). ..

Hybridization:

Article Title: MIDA1, a protein associated with Id, regulates cell growth.
Article Snippet: .. Printed in U.S.A. 24818 at U niv of St A ndrew s on M arch 6, 2015 http://w w w .jbc.org/ D ow nloaded from tured 1% agarose gel, transferred to a nylon membrane, and hybridized with cDNA probe radiolabeled with a random primer extension kit (E. I. du Pont de Nemours & Co., Inc.) in the hybridization buffer (50% formamide, 5 3 SSC, 1 3 FBP (1% Ficol, 1% polyvinylpyrrolidone, 1% bovine serum albumin), 20 mM NaHPO3 pH 6.5, 100 mg/ml of salmon sperm DNA, 10% dextran sulfate, 0.1% SDS). ..

Article Title: Regulatory Function of Delta/YY-1 on the Locus Control Region-like Sequence of Mouse Glycophorin Gene in Erythroleukemia Cells
Article Snippet: .. Ten mg of RNA from each sample were electrophoresed in a denatured 1% gel agarose gel, transferred to a nylon membrane, and hybridized with a mouse glycophorin cDNA probe (1) radiolabeled with a random primer extension kit (DuPont) in the hybridization buffer (50% formamide, 5 3 SSC, 1 3 Denhardt’s reagent, 20 mM NaHPO3, pH 6.5, 100 mg/ml salmon sperm DNA, 10% dextran sulfate, 0.1% SDS). ..

other:

Article Title: Isolation of an elicitor-stimulated 5-epi-aristolochene synthase gene (gPEAS1) from chili pepper (Capsicum annuum)
Article Snippet: Genetic Engineering Department, CINVESTAV-IPN, Unidad Irapuato, Km.. 9.6 Libramiento Norte Carretera Irapuato-León, P.O.. Box 629, C.P.

Activity Assay:

Article Title: Abdominal surgery reduces the ability of rat spleen cells to synthesize and secrete active tumour necrosis factor-alpha (TNF-?) by a multilevel regulation
Article Snippet: Murine TNF-α oligomer (Clontech Labs Inc., Palo Alto, CA), which was 5′-end labelled with (γ- 32 P)ATP using Polynucleotide kinase (Boehringer, Mannheim, Germany) to a specific activity of about 5 × 10 8 ct/min per μg, or β-actin cDNA probe, which was digested with HindIII and the 2.0-kb fragment, was labelled with (α- 32 P)dCTP using the random primer extension kit (DuPont NEN, Boston, MA). .. Murine TNF-α oligomer (Clontech Labs Inc., Palo Alto, CA), which was 5′-end labelled with (γ- 32 P)ATP using Polynucleotide kinase (Boehringer, Mannheim, Germany) to a specific activity of about 5 × 10 8 ct/min per μg, or β-actin cDNA probe, which was digested with HindIII and the 2.0-kb fragment, was labelled with (α- 32 P)dCTP using the random primer extension kit (DuPont NEN, Boston, MA). .. The membranes were autoradiographed with Kodak XAR film in the presence of intensifying screens and exposed at −70°C for 2–14 days.



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